Welcome at Research Microscopy Center LuciaOptics

We are a non profit organisation in the field of super resolution microscopy

We develop novel super resolution microscopy technologies for simpler, faster and more reliable use in molecular biology, medicine, pharmacology and material sciences

Review of biomedical applications in Superresolution Microsopy 2015

Global Engage´s 2nd Microscopy Congress

Day Two – Tuesday 15th November 2016, 09.00-09.35
Keynote Address: Christoph Cremer, Professor, Group Leader, Super Resolution Microscopy, Institute of Molecular Biology, University of Mainz; Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Germany


Super-Resolution Light Microscopy: Challenges for Medical Research
Using super-resolution microscopy (SRM) approaches, both optical resolution (smallest distance measureable between two adjacent point sources) and structural resolution (smallest structural detail determined) can be enhanced very substantially. Here, we shall review some examples of the present state of the art of SRM in medical research and discuss the challenges still to be met to introduce such SRM technologies on a broader scale in medical applications, such as large field of view imaging; long working distances compatible with super-resolution of 3D extended objects; “nanoimaging” of multiple molecular target types (“multicolor SRM”); high throughput SRM to register thousands of cells in a short time, e.g. for tissue diagnostics or pharmaceutical research; as well as the development of fast & reliable data evaluation modes (“Instant Nanoimaging“).

 

Global Engage´s 2nd Microscopy Congress

Micropscopy symposium “Imaging Techniques in Cell Biology – From single molecules to subcellular departments” at LIN Leibniz Institute for Neurobiology Magdeburg, Sep 30,2016

 

Our co-founder Christoph Cremer talks about Super-resolution light microscopy of nuclear nanostructures:

 

LIN Symposium

Structured Illumination microscopy - Spatially Modulated Illumination 

SMI (spatially modulated illumination) microscopy is a light optical process of the so-called point spread function Engineering. These are processes that modify the point spread function (PSF) of a microscope in a suitable manner to either increase the optical resolution, to maximize the precision of distance measurements of fluorescent objects that are small relative to the wavelength of the illuminating light, or to extract other structural parameters in the nanometer  range.more

 

References

  • R. Heintzmann, C. Cremer (1999) Lateral modulated excitation microscopy: Improvement of resolution by using a diffraction grating. Proc. SPIE 3568: 185–196
  • US patent 7,342,717, filed 10th july 1997: Christoph Cremer, Michael Hausmann, Joachim Bradl, Bernhard Schneider Wave field microscope with detection point spread
    function
  • Reymann, J; Baddeley, D; Gunkel, M; Lemmer, P; Stadter, W; Jegou, T; Rippe, K; Cremer, C et al. (2008). "High-precision structural analysis of subnuclear complexes in fixed and live cells via spatially modulated illumination (SMI) microscopy". Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 16 (3): 367–82. doi:10.1007/s1 MID 18461478.
  • Best G, Amberger R, Baddeley D, Ach T, Dithmar S, Heintzmann R and Cremer C (2011). Structured illumination microscopy of autofluorescent aggregations in human tissue. Micron, 42, 330–335

 

New hope for brain attack and heart attack patients

 

This is the first molecular insight into the effect ischemia has on the nuclear architecture of cells. On that basis new pharmaceutical drugs against this major widespread diseases can be developed.

 

Hypoxia (Cellular oxygen insufficiency) occuring in diseases like tumour growth, stroke and cardiac infarction.

Stroke (brain attack) is the second most frequent cause of death after coronary artery disease accounting for 6.4 million deaths (12% of the total).

Cardiac infarction (heart attack), one of the complications of coronary artery disease has with a world-wide incidence rate of 3 - 4 million.

 

"A transient ischemic environment induces reversible compaction of chromatin"

Kirmes et. al, 2015 (Christoph Cremer & George Reid labs IMB Mainz)

 

Histology award for our board member Thomas Cremer

Marion and Thomas Cremer receive the Wilhelm Bernhard Medal

The international Society for Histochemistry announces that the 2015 Wilhelm Bernhard Medal goes to Marion and Thomas Cremer for their joint research on nuclear structure and function

 

 

1. time: Meiosis - super resolution image

First super resolution microscopy images of meiosis

Meiosis - for the first time super resolution microscopy has been used to image this most important step of development of individuals.

 

"Super-resolution imaging reveals structurally distinct periodic patterns of chromatin along pachytene chromosomes",

Prakash et al., 2015 (Cremer-Lab)

 

Experiments done in mouse cells

 

Come to our inventors` talks at the FOM:

Monday, March 30 MO-MO-PAR-A Super Resolution I: Novel working principles and methods (I) (Forum 1), 11:00 - 11:40

Three-dimensional spectral precision distance microscopy (SPDM): Principle and methods 11:00 - 11:20
C. Cremer [invited] (Institute of Molecular Biology (IMB), Germany)

Spectral precision distance microscopy (SPDM) of nuclear spatial organization 11:20 - 11:40
C. Cremer [invited] (Institute of Molecular Biology (IMB), Germany)

 

Tuesday, March 31 TU-AF2-PAR-F Biol. IV: Visualization of dynamics in the cell nucleus (Forum 6), 16:50 - 17:50

Photoconversion of UV excited DNA-binding dyes, and their applications in super-resolution fluorescence microscopy of the cell nucleus 16:50 - 17:10
D. Żurek-Biesiada, P. Waligórski, A.T. Szczurek, U. Birk, Ch. Cremer & J.W. Dobrucki (Jagiellonian University, Poland)

Farfield fluorescence microscopy of nuclear chromatin at molecular optical resolution 17:10 - 17:30
U.J. Birk & C. Cremer (Institute of Molecular Biology (IMB), Germany)

Superresolution imaging of chromatin landscape in meiosis prophase I

17:30 - 17:50
K. Prakash, G. Best, S. Redl, M. Borsos, K. Tachibana-Konwalski, S. Parekh, R. Ketting, U. Birk & C. Cremer (Institute of Molecular Biology (IMB), Mainz Germany, Germany)


Poster P1-B Posters 1-B: Superresolution microscopy I

[P1-B/12] Single molecule localisation microscopy of the distribution of chromatin nanostructures using standard fluorescent probesA.T. Szczurek, K. Prakash, H.-K. Lee, D.J. Żurek-Biesiada, M. Hagmann, G. Best, J.W. Dobrucki, C. Cremer & U. Birk (Institute of Molecular Biology, Germany)

Nobel Prize for Super-Resolved Fluorescence Microscopy

Source: www.nobelprize.org

A former associate of the Cremer Lab, Prof. Dr. Stefan Hell, was awarded the Nobel Prize 2014 in Chemistry for his work on advanced far field light microscopy.

Prof. Hell has published a number of papers on the development and application of advanced optical microscopy techniques together with Prof. Cremer. A particular highlight has been on the development of 4Pi microscopy; the basic concept of this "focused nanoscopy" method was already conceived in the 1970s by the brothers Thomas and Christoph Cremer [C. Cremer, T. Cremer (1978)].

Joint publications Cremer / Hell (1994 - 2014)

Måns Ehrenberg, Member of the Nobel Committee for Chemistry: „This development is expected to revolutionize biology and medicine by, not the least, eventually allowing for realistic, quantitative descriptions at nano-scale resolution of the dynamics of the complex, multidimensional molecular biological processes that define the phenotypes of all life forms.”

Quantitative description at nano-scale resolution in a cell

Quantitative single molecule analysis in a cancer cell - super-resolved fluorescence microscopy with standard fluorescent proteins (C. Cremer), Chromosome (Cooperation C. Cremer/ J. Gall)

18th International Microscopy Congress Prague

Prof. Christoph Cremer invited as plenary speaker

 

WELCOME TO IMC 2014

The Czechoslovak Microscopy Society has the pleasure to invite you to Prague in September 2014. The International Microscopy Congress 2014 will provide the biggest international platform for sharing knowledge, exchange of views and experience, and discussion on all aspects of microscopy.

Imaging synaptic contacts by SPDM


Super-resolution light microscpy and nanoscopy imaging

 

Slot A, 10 September, Small Hall, 14:15 - 14:45

Christoph Cremer: "Superresolution Light Microscopy of Nuclear Genome Organization (invited)

20 Jahre Laborjournal

Vorstoß in atomare Dimensionen

von Christoph Cremer, Mainz/Heidelberg

 

Die Nanoskopie kann ­inzwischen Objekte auflösen, die nur wenige Nanometer auseinander liegen. Bald dürfte auch diese Grenze fallen.