Diseases -  Super Resolution Microscopy

Cancer

Bresast Cancer HER-2/3

Neuroblastoma

 

Blood-Brain Barrier

Relevance for pharmacology

Hypoxia

Stroke

Heart attack

Biological Applications of Super Resolution Microscopy

Histons

Gene transcription

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Neurons

Cellular networks

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Special Applications of Super Resolution Microscopy

Diagnostic Ophtal-mology

Auto-fluorescence

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Microbial Applications of Super Resolution Microscopy

Bacteria

Virus

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Biotechnological and medical applications                            of super resolution microscopy

P-glycoprotein (Pgp; also known as MDR1, ABCB1) is the most important and best studied efflux transporter at the blood-brain barrier (BBB). Immortalized human cerebral microvascular endothelial cells (hCMEC/D3) served as a model of human BBB.hCMEC/D3 cells were transduced with a Pgp-green fluorescent protein (GFP) fusion protein incorporated in a lentivirus-derived vector.



Using SPDMlocalization microscopy it could be shown that Pgp-GFP is distributed inclustered formations inhCMEC/D3 cells while the influence of present random cell membrane curvaturescan be excluded based on the simulation results. Individual formations are distributed randomly over the cell membrane.



Olga Huber,Alexander Brunner, Patrick Maier, Rainer Kaufmann, Pierre-Olivier Couraud, Christoph Cremer, Gert Fricker

Published on September 12, 2012

PLOSone

http://tinyurl.com/bvr2w9d




Our super resolution microscopy technologies can be used in all fields of biomolecular research, including diagnostic and pharmaceutical approaches where single molecule detection in high densities or the detection of sub-diffraction structures is necessary

  • Cancer Research
  • Macular degeneration
  • Signal transduction
  • Stem cell research
  • Cardiology
  • Virus detection
  • Pharmaceutical substances distribution
  • ...
3D Co-localisation super resolution microscopy

Super Resolution Microscopy

www.luciaoptics.org