Super Resolution Microscopy Opthalmology

Macular degeneration

AMD age-related macular degeneration is a medical condition which usually affects older adults and results in a loss of vision in the center of the visual field (the macula) because of damage to the retina Macular degeneration.


Sections from human eye tissue were analyzed with Structured Illumination Microscopy (SMI) using a specially designed Super Resolution Microscope setup (LSI-TIRF). This SMI technique allowes to acquire light-optical images of autofluorophore distributions in the tissue with previously unmatched optical resolution. Use of three different excitation wavelengths (488, 568 and 647 nm) enables to gather spectral information about the autofluorescence signal.



  • Thomas Ach, Gerrit Best, Sabrina Rossberger, Rainer Heintzmann, Christoph Cremer, Stefan Dithmar (2012) British Journal of Opthalmology, BJO Online First, published on July 3, 2012 as 0.1136/bjophthalmol-2012-301547-012-301547
  • A. Brunner, G. Best, R. Amberger, P. Lemmer, T. Ach, S. Dithmar, R. Heintzmann, C. Cremer: Fluorescence Microscopy with Structured Excitation Illumination, in Handbook of Biomedical Optics (D.A Boas, C. Pitris, N. Ramanujam, Edits.), CRC Press, Taylor and Francis, Chapter 26, pp 543 -560, Taylor & Francis, Florida
  • Best G, Amberger R, Baddeley D, Ach T, Dithmar S, Heintzmann R and Cremer C (2011). Structured illumination microscopy of autofluorescent aggregations in human tissue. Micron, 42, 330-335.