Nobel Prize awarded to Prof. Dr. Stefan Hell
A former associate of the Cremer Lab, Prof. Dr. Stefan Hell, was awarded the Nobel Prize 2014 in Chemistry for his work on advanced far field light microscopy. Three
scientists share the award: Two Scientists from USA, Prof. Eric Betzig and Prof. William Moerner, for developing single molecule microscopy using photoactivable proteins, and Prof. Stefan Hell from
Germany, who has been awarded for the development of Stimulated Emission Depletion Microscopy, a "focused nanoscopy" method. (Scientifc background given at www.nobelprize.org
Prof. Hell has published a number of papers on the development and application of advanced optical microscopy techniques together with Prof. Cremer. A particular
highlight has been on the development of 4Pi microscopy; the basic concept of this "focused nanoscopy" method was already conceived in the 1970s by the brothers Thomas and Christoph Cremer [C.
Cremer, T. Cremer (1978), see below].
Some joint publications on 4Pi and STED microscopy:
- S. W. Hell, S. Lindek, C. Cremer, and E. H. K. Stelzer, "Measurement of 4pi-confocal point spread function proves 75 nm axial resolution," Appl Phys Lett, vol. 64,
no. 11, pp. 1335-1337, 1994. pdf
- S. W. Hell, E. H. Stelzer, S. Lindek, and C. Cremer, "Confocal microscopy with an increased detection aperture: type-B 4Pi confocal microscopy," Opt. Lett., vol.
19, no. 3, pp. 222-224, 1994. pdf
P. E. Hänninen, S. W. Hell, J. Salo, E. Soini, and C. Cremer, "Two-photon excitation 4Pi confocal microscope: Enhanced axial resolution microscope for biological
research," Appl Phys Lett, vol. 66, pp. 1698-1700, 1995. pdf
- M. Schrader, F. Meinecke, K. Bahlmann, M. Kroug, C. Cremer, E. Soini, and S. W. Hell, "Monitoring the excited state of a fluorophore in a microscope by
stimulated emission," Bioimaging, vol. 3, no. 4, pp. 147-153, 1995. pdf
- S.W.Hell, M. Schrader, K. Bahlmann, F. Meinecke, C. Cremer (1999) Realisation of depletion by stimulated emission in fluorescence microscopy. In: Focus on Multidimensional
Microscopy Vol.I (P.C. Cheng, P.P. Hwang, J.L. Wu, G. Wang and H. Kim, Edits.), pp.78 – 88. World Scientific Publ. Co., Singapore, New Jerseay, London, Hongkong. Doi:
- M. Lang, T. Jegou, I. Chung, K. Richter, S. Münch, A. Udvarhelyi, C. Cremer, P. Hemmerich,
J. Engelhardt, S. W. Hell, and others, "Three-dimensional organization of promyelocytic leukemia nuclear bodies," J. Cell Sci., vol. 123, no. 3, pp. 392-400, 2010. pdf
For more information on the history of superresolution fluorescence microscopy see
C. Cremer, B.R. Masters (2013) Resolution enhancement techniques
in microscopy. Eur. Phys. J. H 38: 281-344. Direct link to pdf
Publications of the Cremer lab explicitly mentioned in the justification of the nobel prize:
- C. Cremer and T. Cremer (1978) "Considerations on a laser-scanning-microscope with high resolution and depth of field," Microsc. Acta. 81: 31-44. pdf
- R. Heintzmann and C. Cremer (1999),"Lateral modulated excitation microscopy: Improvement of resolution by using a diffraction grating," Proc. SPIE. 3568:185-195.