P-glycoprotein (Pgp; also known as MDR1, ABCB1) is the most important and best studied efflux transporter at the blood-brain barrier (BBB). Immortalized human cerebral microvascular endothelial cells (hCMEC/D3) served as a model of human BBB.hCMEC/D3 cells were transduced with a Pgp-green fluorescent protein (GFP) fusion protein incorporated in a lentivirus-derived vector.
Using SPDMlocalization microscopy it could be shown that Pgp-GFP is distributed inclustered formations inhCMEC/D3 cells while the influence of present random cell membrane curvaturescan be excluded based on the simulation results. Individual formations are distributed randomly over the cell membrane.
Olga Huber,Alexander Brunner, Patrick Maier, Rainer Kaufmann, Pierre-Olivier Couraud, Christoph Cremer, Gert Fricker
Published on September 12, 2012
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